FLUOROCULT LAURYL SULFATE BROTH (LST MUG MEDIUM) MERCK RENDIMENTO 36,5g/l (CALDO LAURIL SULFATO C/MUG) EMBALAGEM GR. Obs.: Mesmo procedimento utilizado para o caldo Brila, porém utilizou-se o meio da marca MERCK, que estimula 37 gramas para 1 litro de. Los hongos se observan en nuestras clínicas teídas con la tinción de Gram como grampositivos. y añadir una gota de tinta china.. Caldo brila. coliformes.

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November 28, ; Returned to authors for corrections: July 23, ; Approved: It is based on reactions of specific microbial enzymes or indicator nutrients of the medium.

There was a significant correlation between the results obtained by the two methods for total coliforms r: In addition, results were available as early as 24h.

The group of total coliforms is the main indicator of the microbiological quality of water and food 7 and their presence indicates improper treatment or post-disinfecting contamination. Its use for water analysis was suggested in 3.

In the milk industry, coliform counts are used to indicate hygiene in milk production and post-pasteurization contamination 9. These methods are laborious and the results for total coliforms are obtained only after 48h incubation, while a further period of 48h incubation is needed to check for the presence of faecal coliforms 8.

These time periods are incompatible with the perishable nature of milk. This system is based calvo enzymes that are not part of the microorganisms. The culture medium offers two active combined substrates, 5-Bromo-4ChloroIndolyl b-d-galactopyranoside X-GAL and the 4-methylumbelliferyl-b-d-glucuronide MUGcaodo simultaneously detect bacteria from the coliform and E.


The total coliforms produce the b-galactosidase enzyme which cleaves the X-GAL releasing the indicator that produces a change of color in the medium from yellow to greenish brika.

For plating, serial decimal dilutions 1: Standard methodology for total and faecal coliform ca,do Total coliforms. The multiple tube technique, using 3 dilutions in triplicates, was used following Brasil – MARA 1with readings after 24h and 48h of incubation. The tubes with blurred medium and gas formation in the Durhan tubes were considered positive.


Samples that fermented lactose in the EC broth gas formation in the Durhan tubes and produced indol in the Tryptone broth were considered positive.

The dehydrated culture medium was dissolved in mL of bi-distilled sterilized water and distributed in sterile test tubes 9. Escherichia coli The tubes that were positive for total coliforms were observed for fluorescence at UV light nm and for production of indol after addition of Kovacs reagent Laborclin, Curitiba, PR, Brazil. Positivity for both tests indicated a positive result for E. The MPN of E.

Statistical analysis The MPN results were converted to log 10 and submitted to statistical evaluation using Excell 97 and Statistica. The Kappa test 6 and a probabilistic model 2,5 were used. The exact concentration of added E. Among 66 samples with counts for total coliforms, in 37 The correlation r between the methods was 0.

The coincidence of these results was Other methods, using the same substrates, were evaluated clado Venkateswaran et al. In 11 samples 8. The correlation between the two methods was 0.



Due to the low number of samples that were positive for faecal coliforms and E. Any alteration to greenish blue or similar tones indicated a positive result. The opacity of the milk did not interfere in color production or fluorescence. Results are easily visualized, and the time required for MPN results for both groups of microorganisms is only 24h.

No false positive or false negative results were observed. A coefficient of agreement for nominal scale. Education and Psychological Measurement Evaluation of a rapid, defined substrate technology method for enumeration of total coliforms and Escherichia coli in chlorinated drinking water. Comparison of a screening test and a birla test in epidemiologic studies.

porém utilizou-se o meio da marca MERCK

The measurement of observer agreement for categorical data. Comparison of dry culture medium and conventional plating techniques for enumeration of bacteria in pasteurized fluid milk. Comparison of comercialy Escherichia coli in foods.

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